Abstract:This paper discusses cyberinformation studies of the nucleotides composition in digital correlation of the methylation of BRCA1 and RASSF1A in EOC tissues, in particular the identification of scientific terminology that could describe this phenomenon, ie, the study of genetic information, as well as the relationship between the genetic language of nucleotides DNA and theoretical aspect of this system and cybernetics. The result of this research show that there is a matrix code in this digital correlation. It also shows that the coding system within the nucleotides language gives detailed information, not only on the DNA „record“, but also on its structure, configuration and its various shapes. The issue of the existence of a digital correlation of the methylation of BRCA1 and RASSF1A code and coding of the individual structural elements of these sequences are discussed. Answers to the following questions are sought. Does the matrix mechanism for biosynthesis of the methylation of BRCA1 and RASSF1A function within the law of the general theory of information systems, and what is the significance of this for understanding the genetic language of RASSF1A and BRCA1 gene? What is the essence of existence and functioning of this language?
Is the genetic information characterized only by biochemical, or also by cyberinformation principles? The potential effects of physical and chemical, as well as cybernetic and information ptinciples, on the biochemical basis of RASSF1A and BRCA1 gene are also investigated.This paper discusses new methods for developing genetic technologies, in particular more advanced digital technology based on programming, cybernetics, and informational laws and systems, and how this new technology could be useful in medicine, bioinformatics, genetics, biochemistry, and other natural sciences.
Keywords: Cancer, Suppressor gene; RASSF1A; BRCA1; Methylation, Unmethylation
Bio code; Genetics Code;
The biologic role of any given RASSF1A and BRCA1 gene in essential life processes depends on the positioning of its component nucleotides, and is understood by the „positioning of letters forming words“. Each of these words has its biochemical base. If this base is expressed by corresponding discrete numbers, it can be seen that any given base has its own program, along with its own unique cybernetics and information characteristics.
Indeed, the sequencing of the molecule is determined not only by distin biochemical features, but also by cybernetic and information principles. For this reason, research in this field deals more with the quantitative rather than qualitative characteristcs of genetic information and its biochemical basis. For the purposes of this paper, specific physical and chemical factors have been selected in order to express the genetic information for RASSF1A and BRCA1 gene. Numerical values are them assigned to these factors, enabling them to be measured. In this way it is possible to determine oif a connection really exists between the quantitative ratios in the process of transfer of genetic information and the qualitative appearance of the RASSF1A and BRCA1 gene. To select these factors, preference is given to classical physical and chemical parameters, including the atomic numbers in the relevant nuc leotides, their analog values, the position in these nucleotides in the genes, and their frenquencies.There is a arge numbers of these parameters, and each of their gives important genetic information. Going through this process, it becomes clear that there is a mathematical relationship between quantitative ratios and the qualitative appearance of the biochemical „genetic processes“ and that there is a measurement method that can be used to describe the biochemistry RASSF1A and BRCA1 gene.
RASSF1A and BRCA1 gene, can be represented by two different forms, ie, a discrete form and a sequential form.
In the discrete form, a RASSF1A and BRCA1 gene is represented by a set of discrete codes or a multiple dimension vector. In the sequential form, a RASSF1A and BRCA1 gene is represent by a series of nucleotides according to the order of their position in the DNA.
Therefore, the sequential form can naturally reflect all the information about the sequence order and lenght of a RASSF1A and BRCA1 gene. The key issue is whether we can develop a different discrete method of representing a RASSF1A and BRCA1 gene that will allow accomodation of partial, if not all sequence order information? Because a RASSF1A and BRCA1 gene sequence is usually represented by a series of nucleotides should be assigned to these codes in order to optimally convert the sequence order information into a series of numbers for the discrete form representation?
Expression of RASSF1A and BRCA1 gene
The matrix mechanism of RASSF1A and BRCA1 gene, the evolution of biomacromolecules and, especially, the biochemical evolution of RASSF1A and BRCA1 gene language, have been analyzed by the application of cybernetic methods, information theory and system theory, respectively. The primary structure of a RASSF1A and BRCA1 gene is the exact specification of its atomic composition and the chemical bonds connecting those atoms.
Table 1 Primer sequences of methylation-specific polymerase chain reaction (Baldwin et al., 2000; Wong et al., 2002)